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Splitting and preparing the donor tissue within an eye bank will improve tissue validation and donor tissue availability and may increase surgeon efficiency. In our report, we present a suspected case of donor-derived Acanthamoeba keratitis after deep anterior lamellar keratoplasty. To the authors' knowledge, there have been no confirmed cases of Acanthamoeba keratitis transmission through corneal transplantation. Deep anterior lamellar keratoplasty was performed on the right eye of a 33-year-old man with severe bilateral keratoconus an

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