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The MICs and MBCs, respectively, for H. sabdariffa extracts had been between 5-20 and 10-20 mg/mL; and for chlorhexidine, between 3-4 and 3-5 mg/mL. For the Listerine®, the MIC and MBC values were between 20-25 and 25-33 mg/mL, respectively. The outcome of this crystal violet test suggest that H. sabdariffa calyx extracts and chlorhexidine affect the permeability of the microbial membrane layer. All H. sabdariffa extracts and chlorhexidine showed significantly better an

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