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Restriction endonucleases (ENases) and DNA methyltransferases (MTases) are very important enzymes in biological processes, and recognition of ENases/MTases activity is considerable for biological and pharmaceutical studies. But, offered nonamplification techniques with a versatile design, desirable sensitivity, and sign production mode of impartial measurement toward numerous nucleases are uncommon. By combining intentionally designed hairpin DNA probes because of the coloca

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