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Sample size was estimated at 24 individuals per group. The DRT group decreased resting heart rate by 5 bpm (  = .03) and increased variance (  = .04) and mean R-R interval (  = .03). The RMT + DRT group decreased mean R-R interval (  = .02). The design for this study appears to be feasible for evaluating manual-therapy intervention in the nonmusculoskeletal function of patients with COPD. It was possible to determine the sample size for future studies. Preliminary data show that the diaphragm release technique may red