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In the forest of Northern Hemisphere, the fungi Heterobasidion annosum sensu lato cause severe root and stem rot diseases, dramatically reducing the wood quality of conifer trees. The hallmark of the host response during the infection process is the formation of necrotic lesions and reaction zones. To characterize physiochemical and molecular features of the necrotic lesion, we conducted artificial inoculations on Norway spruce plants at different developmental stages seedlings, young and mature trees. The results were further compared a

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