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The final method provided an analytical measurement range from 7.8 to 350 nmol/L for PLP and 3.3-339 nmol/L for PA, total imprecision less then 15% and less then 5% for PLP and PA respectively. Calibration against the NIST standard produced measured values within 3% of NIST assigned PLP values. The use of 4-deoxypyridoxine as internal standard did not improve precision or accuracy when compared to calibration using 5-level external standards. Conclusions This method combines derivatization and protein precipitation in one step and is t

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