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Long noncoding RNAs (lncRNAs) have been widely recognized to play an important role in a variety of diseases. Abnormal regulation of lncRNA GATA3‑antisense RNA 1 (AS1) occurs in several cancers, but whether it is involved in the progression of pancreatic cancer (PC) remains unknown. The present study aimed to investigate the biological effects of GATA3‑AS1 in PC and to explore the underlying molecular mechanisms. Upregulation of GATA3‑AS1 was revealed in PC tissues and cell lines. Knockdown of GATA3‑AS1 in PANC‑1 or AsPC‑1 cells markedl