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We describe a protocol for imaging a mitochondrial fluorescence transient increase event (Mitoflash) in live cardiomyocytes using a confocal microscope. Mitoflash, detected by mitochondria-targeted circularly permuted fluorescent protein (mt-cpYFP), can be used to assess mitochondrial respiration function in situ. The protocol is also suitable for live-cell imaging of other adherent cells, including fibroblasts and hepatocytes. For complete details on the use and execution of this protocol, please refer to Gong et al. (2014) and Gong et a

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https://www.selleckchem.com/products/shikonin.html
https://www.selleckchem.com/products/shikonin.html
Long lasting series stability along with bone tissue marrow tropism within a macaque label of human pegivirus an infection. Nano-silver Fluoride in Higher Awareness pertaining to Caries Charge in Primary Molars: A new Randomized Managed Trial. Eczema herpeticum (EH) is a rare complication of atopic dermatitis (AD) caused by disseminated herpes simplex virus (HSV) infection. The role of rare and/or deleterious genetic varian