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© The author(s).Changes in mitochondrial structure and function are mostly responsible for aging and age-related features. Whether healthy mitochondria could avoid aging is, nonetheless, confusing. Here we intravenously injected the mitochondria isolated from young mice into aged mice and investigated the mitotherapy on biochemistry metabolic rate and animal habits. The outcomes indicated that heterozygous mitochondrial DNA (mtDNA) of both aged and young mouse coexisted in cell