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To explore a method for rapidly screening the Duffy blood group genotypes and to establish an information bank of rare blood type donors. The microfluidic capillary electrophoresis system and PCR-SSP method were used to analyze the Duffy genotype of 3 936 unrelated O-type blood donors in our center from December 2014 to September 2018. The serologic identification and typing of other blood type system phenotypes for FY -negative specimen were performed. The Fy (a-b-) specimen was sequenced for genotyping. The phenotypes of F