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Immune responses must be tightly regulated in order to avoid excessive inflammation. In this issue of Cell Reports, Bambouskova et al. (2021) demonstrate that itaconate and iNOS collaborate to tolerize the NLRP3 inflammasome, thereby limiting cytokine secretion and cell death.We present a sheathless, microfluidic imaging flow cytometer that incorporates stroboscopic illumination for blur-free fluorescence detection at ultra-high analytical throughput. The imaging platform is capable of multiparametric fluorescence quantification and sub