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Different DNA extraction kits and enrichment procedures were investigated to obtain the most practical workflow. We demonstrated the feasibility of shotgun metagenomics to obtain the same information as in ISO/TS 131362012 and WGS of the isolate in parallel by inferring the genome of the contaminant and characterizing it in a shorter timeframe. This was achieved in food samples containing different E. coli strains, including a combination of different STEC strains. For the first time, we also managed to link individual str

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7 %±2.9 % vs. 37.2 %±4.3 %) and prolonged linear release period (20.47 h vs. 4.97 h for releasing 90 % of the loaded drug). Mechanisms by which the shell's lipid layer adjusts the release behavior of TC molecules are proposed. The present protocol based on coaxial electrospraying shows a new strategy of combining edible protein and lipids to fabricate advanced functional nanomaterials.Microfluidic platforms have become highly attractive tools for synthesis of nanoparticles, including lipid nano-self-assemblies, owing to unique features and