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While Uzbekistan and Central Asia are known for the well-studied Bronze Age civilization of Bactria-Margiana Archaeological Complex (BMAC), the lesser-known Iron Age was also a dynamic period that resulted in increased interaction and admixture among different cultures from this region. To broaden our understanding of events which impacted the demography and population structure of this region, we generated 27 genome-wide SNP capture datasets of Late Iron Age individuals around the Historical Kushan time period (∼2100-1500 BP) from thre