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MOTIVATION Motif-HMM (mHMM) scanning has been shown to possess unique advantages over standardly used sequence-profile search methods (e.g. HMMER, PSI-BLAST) since it is particularly well suited to discriminate proteins with variations inside conserved motifs (e.g. family subtypes) or motifs lacking essential residues (false positives, e.g. pseudoenzymes). RESULTS In order to make mHMM widely accessible to a broader scientific community we developed Leitmotif, a mHMM web application with many parametrization options easily accessible thr

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