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Glyphosate-based herbicide (GBH) using is increasing on a global scale. Few studies have investigated the sub-lethal effects of GBH in endemic amphibian species. The present work tested the GBH Roundup Active® on the tadpoles of Dendropsophus molitor. The exposure was in a range of plausible environmental concentrations (0-0.75 μg a.e./L) during a month. D. molitor is an endemic tropical frog of South America. The exposure from 325 μg a.e./L caused histological alterations in the liver. The high-resolution optical microscopy (HROM) detect